Spectrophotometric assay for ornithine decarboxylase
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چکیده
منابع مشابه
A safety catch for ornithine decarboxylase degradation
Feedback inhibition is a common mechanism to adjust the activity of an enzyme in accordance with the abundance of a product. The enzyme catalyzing the initial, committing step of a biosynthesis cascade is subject to negative feedback by the end-product. This kind of regulation is frequent in all cell types to regulate biosynthesis of numerous metabolites; a classical textbook example is the ser...
متن کاملRegulation of mammalian ornithine decarboxylase.
17 Melvin, M. A. L. and Keir, H. M. (1978) Expt. Cell Res. 111,231-236 18 Melvin, M. A. L. and Keir, H. M. (1979) Biochem. SOC. Trans. 7, 689-691 19 Wallace, H. M. and Keir, H. M. (1981) Biochim. Biophys. Acta 676,25-30 20 Melvin, M. A. L., Wallace, H. M. and Keir, H. M. (1980) Physiol. Chem. Phys. 12, 431-439 21 Coleman, C. S. and Wallace, H. M. (1990) 'Biochem. SOC. Trans. 18, 1228-1229 22 Wa...
متن کاملRegulation of Adrenal Ornithine Decarboxylase
were not secondary to stimulation of steroidogenesis, since hydrocortisone had no effect on adrenal ornithine decarboxylase although it did stimulate activity of the enzyme in the liver and kidney. ACTH, given subcutaneously to hypophysectomized rats, induced striking increases in adrenal cyclic AMP levels within 15-30 min with a fall towards the base line in 1 h. Increases in ornithine decarbo...
متن کاملExpression of human chromosome 2 ornithine decarboxylase gene in ornithine decarboxylase-deficient Chinese hamster ovary cells.
Ornithine decarboxylase (ODC) belongs to a multigene family and some of these may very well be nonfunctional (pseudogenes). We isolated an ODC gene from a human chromosome 2-specific library and transfected the gene into ODC-deficient Chinese hamster ovary cells to directly demonstrate that this ODC gene is functional and ODC is essential for cell proliferation. After screening 2.5 X 10(5) plaq...
متن کاملExpression of Human Chromosome 2 Ornithine Decarboxylase Gene in Ornithine Decarboxylase-deficient Chinese Hamster Ovary Cells1
Ornithine decarboxylase (OIK ) belongs to a multigene family and some of these may very well be nonfunctional (pseudogenes). We isolated an ODC gene from a human chromosome 2-specific library and transfected the gene into ODC-deficient Chinese hamster ovary cells to directly demonstrate that this ODC gene is functional and OIK is essential for cell proliferation. After screening 2.5 x 10* plaqu...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1987
ISSN: 0003-2697
DOI: 10.1016/0003-2697(87)90049-2